Vegetal LoFlo Medium Size:6 x 1kg resulting in an increase of

resulting in an increase of cell density up to 5 x 10^7

Yeast extract 10 Peptone 20 Glucose 20 Agar 20 Suspend 70 gram powdered medium in 1 litre distilled water

C6H12O6  180

allowing for the separation of biomolecules based on their size

used to separate and analyse DNA

Vegetal LoFlo Medium Size:6 x 1kg resulting in an increase ofLoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to